A RAPID AND SIMPLE METHOD OF DNA EXTRACTION FROM CLINICAL SPECIMENS CONTAINING MYCOBACTERIUM LEPRAE FOR PCR ASSAYS
نویسندگان
چکیده مقاله:
We describe a rapid, simple, and reliable procedure for routine isolation of Mycobacterium leprae DNA from slit-skin swab specimens. This one-step DNA extraction method is based on the utilization of ChelexR 100, a chelating ion exchange resin. Slit-skin swab specimens from seven untreated leprosy patients at Baba Baghi Leprosy Hospital in Iran were processed by this procedure. The polymerase chain reaction (PCR) was performed with primers for a 530-base pair (bp) fragment of the gene encoding the 36 kDa antigen of M. leprae. All the specimens were found to be PCR -positive, suggesting the efficiency of the present DNA extraction method.
منابع مشابه
a rapid and simple method of dna extraction from clinical specimens containing mycobacterium leprae for pcr assays
we describe a rapid, simple, and reliable procedure for routine isolation of mycobacterium leprae dna from slit-skin swab specimens. this one-step dna extraction method is based on the utilization of chelexr 100, a chelating ion exchange resin. slit-skin swab specimens from seven untreated leprosy patients at baba baghi leprosy hospital in iran were processed by this procedure. the polymerase c...
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عنوان ژورنال
دوره 10 شماره 3
صفحات 233- 235
تاریخ انتشار 1996-11
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